Search results for " adeno-associated virus"
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Recombinant Adeno-Associated Virus Type 5 Production Process
A scalable recombinant adeno-associated virus (rAAV) type 5 production process was developed using transfected HEK293 suspension cells.
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Webcast: Repeatable, automated SPR titer assay for adeno- and adeno-associated viruses in crude samples
Fredrik Sundberg at Cytiva shares our recent SPR assay developments for measuring adenovirus and adeno-associated virus titers.
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Production of Recombinant Adeno-associated Virus Vectors Using Microcarrier Culture of HEK293T Cells
As of March 2020, more than 4310 gene therapy clinical trials have been performed worldwide, with only 518 of them in Phase III and Phase IV. As the gene therapy projects enter clinical Phase III or g…
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Ensuring Viral Safety of Viral Vaccines and Vectors
In adeno-associated virus (AAV) processes, any replication-competent helper adenovirus must be inactivated and removed during downstream purification. AAV particles can resist heating at 52 ⁰C for 1…
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Application of Quality by Design to Viral Safety
For instance, massively parallel sequencing has been used to evaluate bovine serum, and this testing helped distinguish bovine adeno-associated virus (BAAV-2) as a new contaminant (5). Once a panel of…
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Gene Therapies Push Viral Vector Production
By Feliza Mirasol
Several virus types have been studied for use as viral vectors in gene therapy, including adenovirus, lentivirus, retrovirus, and adeno-associated virus (AAV). Of these, AA…
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Scalable Viral Vector Manufacturing
However, as certain types of viruses such as Lentivirus for cell therapy and Adeno-Associated Virus for gene therapy become more common, there is a possibility for development of virus specific platfo…
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Single-Use Technologies Prove Effective for Viral Vector Process Development
Notably for the manufacture of vectors based on adeno-associated virus (AAV), one of the most common vector types for gene therapy delivery and viral-vector vaccines, recent data have shown that stain…
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A Look at the Affinity Chromatography Landscape
A Look at the Affinity Chromatography Landscape
New ligands are being developed to meet the separation and purification needs of next-gen biologics.
By Cynthia A. Challener
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Successful Development of Scalable AAV Processes: Part 2 of 2
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In the second of a two-part series, Anki Magnusson, Staff Research Engineer, Protein and Viral Production, and Åsa Hagner-McWhirter, PhD, Senior Scientist, Bio…